Journal: bioRxiv
Article Title: Towards molecular-based functional classification of fetal bovine serum
doi: 10.64898/2026.03.16.712020
Figure Lengend Snippet: (A) Hierarchical clustering dendrograms of FBS conditions based on differential gene expression profiles in MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Clustering was performed using the number of differentially expressed genes (DEGs; |log2FC| > 0.6, adjusted p-value < 0.05) as a distance metric. Horizontal red lines indicate the clustering threshold of 100 DEGs, defining distinct FBS clusters. MRC-5 and THP-1 cells show 4 distinct clusters, while Jurkat cells display 5 clusters, revealing cell type-specific responses to FBS alternatives. (B) Module size distributions from WGCNA for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Bar colors represent distinct co-expression modules identified using signed networks with Pearson correlation (soft-thresholding power selected to achieve scale-free topology R ≥ 0.8; minimum module size = 30 genes; merge cut height = 0.25). MRC-5 cells: 7 modules (turquoise: 4,728 genes; blue: 3,580; brown: 2,417; grey: 936; yellow: 634; green: 447; red: 234). Jurkat cells: 7 modules (turquoise: 4,234; blue: 3,635; grey: 2,289; brown: 1,464; yellow: 660; green: 240; red: 204). THP-1 cells: 5 modules (turquoise: 4,201; blue: 4,16; grey: 2,478; brown: 2,184; yellow: 434). (C) Module-trait relationship heatmaps showing Pearson correlations between module eigengenes and FBS cluster assignments for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Each cell displays the correlation coefficient (color scale: blue = -0.5, white = 0, red = 0.5) and corresponding p-value. Significant correlations (p < 0.05, calculated using corPvalueStudent) indicate modules with expression patterns specifically associated with FBS clusters. Notable associations include: MRC-5 turquoise module with Cluster 1 (r = - 0.45, p = 3.22×10 ); Jurkat red module with Cluster 2 (r = 0.76, p = 1.38×10 ); THP-1 yellow module with Cluster 1 (r = 0.65, p = 2.54×10 8).
Article Snippet: The adherent human lung fibroblast cell line MRC-5 (CCL-171), the suspension human monocytic cell line THP-1 (TIB-202), and the human acute T-cell leukemic cell line Jurkat Clone E6-1 (TIB-152) were purchased from American Type Culture Collection (ATCC, USA).
Techniques: Gene Expression, Expressing
ATCC is a verified supplier 
ATCC manufactures this product 
